PCR products electrophoresis

I am trying to clearly separate two DNA bands on a gel. The difference
between them is 27 bp, and the overall size is between 100 - 200 bp. I have
tried 5% acrylamide gels, and get very sharp defined bands, but my negative
control lane always looks lousy. The same tube run on a 2% agarose gel
looks much better (ie negative control lane empty) but ... ...

Last update 27-Oct-2001, Rating of 3 votes.

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