Protein Kinase C Assay

The following is a modification of Kikkawa et al., (1982) J. Biol. Chem. 257, 13341. This assay measures phosphorylation (32P) of a protein kinase C-peptide substrate, which is subsequently separated by binding to phosphocellulose paper. (Newton lab, Department of Pharmacology, University of California at San Diego)

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Last update 28-Jan-2005, Rating n/a of 0 votes.