MicroRNA and siRNA Cloning Method

MicroRNA and siRNA Cloning Method (Bartel Lab Protocol, MIT).

Procedural Outline:
1. Oligo Design
2. ImpA Synthesis
3. Synthesizing Oligos
4. Oligonucleotide Adenylation
5. Small RNA Purification
6. 3' Adaptor Ligation
7. 5' Adaptor Ligation
8. RT-PCR
9. Concatamerization
10. Cloning into Topo vector
11. Screen and Sequence

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Last update 30-Jan-2005, Rating n/a of 0 votes.