Removal of genomic DNA contamination in RT-PCR

I have just begun RT-PCR experiments. They are going very well so far
with beautiful amplification as show on agarose gel. However, when I run
the no-RT control I have a very faint band indicating that I have not
fully removed the DNA from my RNA. I measured the amount of RNA/DNA in
my sample and according to the manifacturers notes 30 minutes at 37°C
with 1U DNase should be ample to remove my DNA. Increasing the time does
not seem to help. Has anyone ... ...

Last update 23-Mar-2006, Rating of 1 votes.

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	By Idania on 23-Mar-2006 I am having problems trying to eliminate plasmid DNA from an in vitro transcription reaction. I have tried more than one protocol. Even on-column digestion. Could anyone advice me? Best regards, Idania Rating: Reply

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