Technique / Molecular Biology / RNAi siRNA gene silencing
PCR template method for dsRNA Synthesis (RNAi)
PCR template method for dsRNA Synthesis (RNAi)
A method for synthesis of iRNA which allows both RNA strands to be generated simultaneously from a PCR fragment which contains a T7 promoter on each end. (from Carthew/Clemens/Tuschl, MIT)
Related resource
Rnai: A Guide to Gene Silencing
RNA Interference of Gene Expression (RNAi) in Cultured Drosophila Cells

siRNA design tools from Whitehead Institute

siRNA sequence list (human, rat, mouse)

RNA Silencing Methods and Protocols

RNAi technique animation tutorial from Nature

siRNA transfection

Knock-down of endogenous proteins by RNA interference in HeLa cells

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