Technique / Cytometry and fluorescence microscopy / Fluorescent confocal microscopy
Anti-HA tag Immunocytology
Anti-HA tag Immunocytology
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California)
Protocol from Yale Genome Analysis Center YGAC.
Related resource
How does a confocal microscope work?
General protocol on Immunofluorescence staining

Immunofluorescence Double Staining Method Parallel Approach

Mitochondria staining and Actin Filament double staining protocol

BD Living Colors FAQ's - Troubleshooting

Double immunofluorescence staining for BCL-6 and else

Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells

Fixing and pelleting chromatin/nuclei from extracts onto coverslips for immunofluorescence

Promotions