Technique / Cell Biology / Cell cycle analysis
G1/S Phase Block for Cell Synchronization
G1/S Phase Block for Cell Synchronization
This is a protocol using aphidicolin to synchronize whole culture cells in G1/S phase. The amount of aphidicolin (and the time of exposure) may vary depending on the cell line you are working with. (Andrew Murray, Harvard University)
Last update 01-Feb-2005, Rating of 0 votes.
Related resource
Flow Cytometric Protocols for Cell Cycle Analysis
Best way of cell cycle separation
Cell synchronization protocol
G1/S cell synchnronization using double thymidine block
Cell cycle methods (method booklet)
Cell-cycle independent internal control genes
BrdU and PI staining for cell cycle analysis
Metaphase Block for Cell Synchronization
Promotions