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G1/S cell synchnronization using double thymidine block



G1/S cell synchnronization using double thymidine block

This protocol is designed to synchronize HeLa cells at the G1/S border (actually all G1). It is highly effect and gives almost complete synchronization in the culture. Note that this protocol will not work on cell lines with intact p53 apoptotic responses. (Science Advisory Board)

Last update 25-Oct-2006, Rating of 3 votes.

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By Mirijam on 10-Oct-2008
Have the same problem ...at the moment i am trying to starve them (arrest in G0-G1)...hope it'll work...
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By STP on 25-Jun-2008
Do you have a protocol for synchronising wild-type p53 cells? I'm having difficulty trying to get a pure cell population, have tried shotening the treatment period and it seems to help but I still have 15-20% of the cells in G1 when release from the thymidine block.
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By Helene Z Hill on 25-Oct-2006
Do you have a reference for double thymidine block not working on p53 wild type -- ie only works on mutant p53?
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