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Cell cycle methods (method booklet)



Cell cycle methods (method booklet)

Method booklet on cell cycle analysis (BioSource international).

Content:

Introduction ................................................................................2
I. Cell Proliferation ....................................................................4
Detection of Proliferating Antigens ..............................4
Measuring DNA Synthesis ............................................5
alamar Blue™ Reduction ..............................................5
II. Cell Synchronization ........................................................13
Serum Starvation ........................................................13
Chemical Inhibitors ....................................................13
Propidium Iodide Staining ..........................................15
Intracellular Antigen Detection ..................................17
FITC conjugated................................................................18
Unconjugated ..................................................................19
III. Immunological Methods................................................22
IP ................................................................................25
Western Blot ..............................................................29
IHC ............................................................................40
IV. Regulation of Protein Phosphorylation: Case
Studies ....................................................................................46
Case Study 1: Regulation of Cyclin B1/cdk ................46
Case Study 2: Regulation of Retinoblastoma ..............49

Last update 01-Mar-2005, Rating of 7 votes.

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By Sez on 27-May-2008
i need to find out specific details of the cell cycle protocol. send?
cheers
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By guimin wang on 09-Jan-2008
I need Cell Synchronization protocol. How I can get it?
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By rachu on 27-Apr-2007
i wanted to know how to arrest the cells in different stages of cell division
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By aitmouse on 17-Apr-2007
Hi
I want to know how percent cells in G1, S, G2, M in a normal culture of tumoral cells (exp phase of the culture);
Thanks



g
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By tulasi on 25-Oct-2006
want to know cell cycle protocol
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By choti on 13-Jun-2006
+ Hi
I had a question on cell cycle protocol.
I wanted to study the effect of taxol as to whether the cells go form metaphase before they enter the cell death end point.
For that i needed to synchronize the cells with aphidicolin.
What is the protcol, I should follow.
Please give me some guidance

Thanks

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By siddesh on 01-Mar-2005
nothing special

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