Technique / Histology


Histotechniques



Histotechniques

Content:

  • Tissue Processing
  • Specimen Accessioning
  • Gross Examination
  • Fixation - types of fixatives
    Aldehydes
    Mercurials
    Alcohols
    Oxidizing agents
    Picrates
  • Fixation - factors affecting fixation
    Buffering
    Penetration
    Volume
    Temperature
    Concentration
    Time interval
  • Fixatives - general usage
  • Tissue Processing
  • Sectioning
  • Frozen Sections
  • Staining
  • H and E staining
  • Coverslipping
  • Decalcification
  • Artefacts in Histologic Sections
  • Problems in Tissue Processing

    Last update 03-May-2005, Rating of 4 votes.

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    By John munjalu on 25-Jul-2008
    Well procedurised but try to ellaborate some processes.such as dehydration ,clearing,embedding etc.
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    By hall0 on 17-Mar-2008
    j'ai besoin d'aide pour les techniques immunohistochimiques appliquées au maladies de poisson d'élevage : decentrarchus labrax
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    By kcox on 17-Aug-2006
    I need help with tissue processing. Many of our tissues are under processed. What are the critical times for tissues in 70,95,100% EtOH? Coroner brain specimens seem to be the biggest problem.
    Any suggestions?
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    By Kathy Martino on 03-May-2005
    GREAT WEB SIGHT
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