Technique / Cell Biology / Cell culture / Common cell line culture


Establishing stable cell lines...



Establishing stable cell lines...

I am trying to establish stable mammalian cell lines
expressing a transfected gene by G418 selection in CHO
cells.

I treat the transfected cells with G418 @ 400ug/ml (I
assume this is this enough ?) but ... ...

Last update 12-Mar-2002, Rating of 0 votes.

Post your message in Common cell line culture forum

Write your comment

By Andy on 12-Mar-2002
I am trying to establish a stable mammalian cell line (hek293 cells) transfected with pcDNA3 vector, by using Geneticin as selective agent at 500 microgram/mL for a month or so, then at 200microgram/mL for maintaining. Cells have been growing well until recently they suddenly detached extensively after i splitted them just as usual.

That is really annoying. Could anybody tell me what is going wrong with my cells?

Thanks a lot.
Rating:

Reply


Please Login or Register to Post