Technique / Molecular Biology / RNAi siRNA gene silencing
Targeting genes expressed in mammalian cells using siRNAs
Targeting genes expressed in mammalian cells using siRNAs
A nature pdf protocol and troubleshooting guide on siRNA. (Thomas Tuschl,Laboratory for RNA Molecular Biology, The Rockefeller University)
This is a detailed protocol with troubleshooting solutions in each critical steps.
Related resource
Rnai: A Guide to Gene Silencing
RNA Interference of Gene Expression (RNAi) in Cultured Drosophila Cells

siRNA design tools from Whitehead Institute

siRNA sequence list (human, rat, mouse)

RNA Silencing Methods and Protocols

RNAi technique animation tutorial from Nature

siRNA transfection

Knock-down of endogenous proteins by RNA interference in HeLa cells

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