Technique / Histology / Immunohistochemistry techniques
Immunostaining Protocol with Video Demonstration
Immunostaining Protocol with Video Demonstration
A step by step immunostaining protocol with video demonstration on how to perform immunocytochemistry fluorescent staining on cell cultures on coverslips.
You are recommended to first download their detailed protocol in pdf format including buffer and reagent for immunostaining. Quicktime movie video is also available for download for important steps of the immunostaining technique. ( Angus Lamond's research laboratory, University of Dundee, Scotland)
Last update 18-Jan-2002, Rating of 2 votes.
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By maco
on 02-Feb-2008
please I want some protocol for immunohistochemistry for heat shock protein (Hsp 60 Hsp70) |
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By shashi
on 10-Mar-2007
i want some protocol of immunohistochemisry for rats brains |
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By harsha doddapaneni
on 26-Aug-2003
hai, your streptavidin bead conc seems ok.how long is the spacer arm in your biotin . keep it as long as possible so that there would be minimum steric hinderance in later binding. i think this is critical. i use biotin- 21- dUTP which has 21-atom spacer arm, for 3'-end labelling and incorporate it through PCR. it worked fine in my clone capture procedure. |
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By harsha doddapaneni
on 26-Aug-2003
hai, your streptavidin bead conc seems ok.how long is the spacer arm in your biotin . keep it as long as possible so that there would be minimum steric hinderance in later binding. i think this is critical. i use biotin- 21- dUTP which has 21-atom spacer arm, for 3'-end labelling and incorporate it through PCR. it worked fine in my clone capture procedure. |
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By Muller Maurice
on 13-Aug-2002
GeBAflex-tube recover 80%+ oligo of DNA extraction. |
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By Boukaba
on 18-Jan-2002
Be sure that the salt buffer is 2M other wise the biotin will not bind |
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