Technique / Proteomics and protein biochemistry / Immunoprecipitation


How to remove RNase from immunoprecipitation?



How to remove RNase from immunoprecipitation?

We are trying to do a "simple" immunoprecipitation in which
the unbound fraction will be assayed for RNA processing activity. We find that
protein A-agarose beads have little non-specific RNase activity. After adding
serum (immune or non-immune), we wash the beads up to 10 times until the wash
supernatant has no detectable RNase.


However, when we do the actual IP by incubating these beads with the experimental enzyme fraction, the unbound supernatant has much more non-specific RNase activity ... ...

Last update 03-Dec-2003, Rating of 0 votes.

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By loulou on 03-Dec-2003
i need to know the precedure of how the immunoprecipitation take place, about its technique of this and for what tests does it done. wish that have ur answer coz i searched but i didn't get the accurate answer
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