Technique / Immunology / Assays for lymphocyte function

C7 Isolation of mouse peripheral blood mononuclear cells by flotation


C7 Isolation of mouse peripheral blood mononuclear cells by flotation (archive)

Isolation of PBMC using Axis Shield Density Gradient Media. (Axis Shield inc.)

Standard human peripheral blood mononuclear cell (PBMC) isolation media such as lymphoprep or Nycoprep 1.077 are less effective for the isolation of these cells from the blood of certain experimental animals. The density of the PBMCs from mice, rats and rabbits is apparently slightly higher than that from humans. Some commercial media simply address this problem by having a correspondingly raised density. This simple solution however fails to address the simultaneous problem that the density of the polymorphonuclear leukocytes (PMNs) is the same. Thus although recoveries of PBMCs are satisfactory, contamination from PMNs can be significant.

Last update 02-Dec-2005, Rating Very Good of 5 votes.

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By carolina castillo on 26-Jul-2008
me gustaria saber sobre la produccion de citoquinas proinflamatorias por las celulas mononucleares de la sangre periferica
Rating: Very Good

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By J Fang on 28-Feb-2008
Can you send me more information about isolation of mouse peripheral blood mononuclear cells?
Rating: Excellent!

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By enrique on 28-Jun-2007
me gustaria que me ayude en el tema de utlizar el percoll para aislar celulas mononucleares como poder variar su densidad es decir igualarla al de ficoll

Rating: Excellent!

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By M.PALANISAMY on 28-Nov-2006
its very usefull.but i want to know about more detais for this type of isolation
THANKYOU
Rating: Excellent!

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By Izabela Jatczak on 02-Dec-2005
Good morning,
Could You send me some more information about C7 Isolation of mouse peripheral blood mononuclear cells by flotation? Maybe do you have other kit for isolationing of PBMC from mice.
Thank you very much,
Best wishes,
Iza
Rating: n/a

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