Detection of mutations by RNAse cleavage (archive)
Protocol from Great Ormond Street Children's Hospital.
This method is based on the fact that many ribonucleases will cleave single stranded RNA and therefore will cleave DNA:RNA or RNA:RNA hybrids in heteroduplexes where there is a mismatched base. Cleavage of RNA is thought to occur 3กฏ to pyrimidines and of the 12 possible types of mismatches, four are recognized more efficiently (C:A, C:C, C:T, U:T). The sequence surrounding the mismatch may be important in determining the efficiency of cleavage.
Last update 02-Mar-2005, Rating n/a of 0 votes.
