Technique / Molecular Biology / PCR / Quantitative real-time PCR
Real time PCR
Real time PCR
We are planning to have a new Real Time PCR machine in our lab but still in
puzzle to find the best. Can anyone ... ...
Last update 01-Mar-2002, Rating of 0 votes.
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By Maria
on 01-Mar-2002
I am performing a quantitation of the mRNA levels of several stress genes in a group of yeast strains, with RT and subsequent real time PCR. As standard samples (to construct the standard plot) I use always the same cDNA with serial dilutions. The problem: the efficiency of the PCR reaction and, hence, the slope of the standard curve varies from one experiment to another. So, I cannot compare the levels obtained in different experiments. |
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