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PCR contamination: amplification in negative control

PCR contamination: amplification in negative control

PCR product band is observed in agarose gel electrophoresis from a negative control.

1. Work in a pre-PCR workspace ( i.e. PCR station with UV light) to avoid contamination from previously amplified DNA
2. Put on a fresh pair of gloves when begining work PCRs. Change gloves frequently.
3. Prepare your own sets of reagents and store them in small aliquots. When preparing these reagents, use RNase? DNase free and steriled tubes.
4. It is best to add all components of the reaction to the microfuge tube before adding the template DNA.
5. Whenever possible, include a positive control as well as a negative control that contains all the components of the PCR except the template DNA.

Last update 28-Feb-2002, Rating Fair of 6 votes.

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