A Practical Guide to Microarray Analysis of Gene Expression

A comprehensive practical guide and protocol for microarray analysis written by Mamdouh Aldamassi, Zugen Chen, Barry Merriman, Daniel Gussin and Stan Nelson at UCLA microarray core facility.

The goal of this document is to provide a brief, practical guide for doing gene expression analysis of tissues and cell lines, using spotted cDNA microarrays and two-color fluorescent labeled comparative probes.

Content:

• cDNA microarray fabrication
• RNA extraction from tissues or cells
• Fluorescent labeling of RNA probe
• Hybridization of probe to the microarray
• Washing off of unbound probe
• Data acquisition by fluorescent scanning of the array
• Data extraction from the scanned image
• Data normalization
• Basic analysis techniques for gene expression data

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Last update 24-Mar-2005, Rating n/a of 0 votes.