Technique / Proteomics and protein biochemistry / Immunoprecipitation
General Principles of Immunoprecipitation
General Principles of Immunoprecipitation
This is the Bart's cookbook on immunoprecipitaiton. It gives some general principles on performing immunoprecipitation using different lysis buffers.(Sefton Lab, Salk Institute).
Content:
RIPA buffer
NP40 buffer
PBS
TN
Boiling in SDS
Antisera
Bugs
Secondary Antibodies
Cell Lysis
Pre-clearing
Tamara' s abbreviated protocol
Last update 08-Feb-2006, Rating of 7 votes.
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By jack
on 28-Jun-2007
dear sir |
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By yasin daraweesh
on 09-May-2007
dear sir, |
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By Syeda Zainab Fatima
on 04-Jan-2007
i wanted to know the principle behind ouchterlony's double immunodiffusion method. |
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By karthikeyan
on 31-Oct-2006
respected sir, |
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By Vijay Chaitanya
on 24-Jun-2006
Dea sir, |
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By J.Heerkens
on 01-Mar-2006
I am immunoprecipitating a phosphorylated enzyme, but in the negative control we get exactly the same strong band as in the sample. The negative control we use is where we do not add antibody to the beads, only the homogenate. Is this only due to non-specific binding or is it possible the homogenate precipitates and goes along with the beads all the time? |
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By pushpinder
on 08-Feb-2006
dear sir, |
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