Technique / Proteomics and protein biochemistry / Immunoprecipitation


General Principles of Immunoprecipitation



General Principles of Immunoprecipitation

This is the Bart's cookbook on immunoprecipitaiton. It gives some general principles on performing immunoprecipitation using different lysis buffers.(Sefton Lab, Salk Institute).

Content:

RIPA buffer
NP40 buffer
PBS
TN
Boiling in SDS
Antisera
Bugs
Secondary Antibodies
Cell Lysis
Pre-clearing
Tamara' s abbreviated protocol

Last update 08-Feb-2006, Rating of 7 votes.

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By jack on 28-Jun-2007
dear sir
I want to know the RIPA buffer for extract cell protein
thanks
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By yasin daraweesh on 09-May-2007
dear sir,
i want to prepare presentain about immunoprecipitation, can you please provide me with good details and information /enemenation
thanks
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By Syeda Zainab Fatima on 04-Jan-2007
i wanted to know the principle behind ouchterlony's double immunodiffusion method.
thanks
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By karthikeyan on 31-Oct-2006
respected sir,
i was working for an cancer i planned to work under immunoprecipitation follow to this i also do immuno histochemistry and western blotting as i am new to do this techniques can you please provide me the details and protocol about all these techniques with the materials.
thank you
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By Vijay Chaitanya on 24-Jun-2006
Dea sir,
I want to perform co immunopecipitation . can you please provide me with a good protocol and the details of the experiment?
Thanking you,
G.VIjay chaitanya
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By J.Heerkens on 01-Mar-2006
I am immunoprecipitating a phosphorylated enzyme, but in the negative control we get exactly the same strong band as in the sample. The negative control we use is where we do not add antibody to the beads, only the homogenate. Is this only due to non-specific binding or is it possible the homogenate precipitates and goes along with the beads all the time?
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By pushpinder on 08-Feb-2006
dear sir,
i am new to do immunoprecipitation, how can i do immunoprecipitation and then wetern blotting, i have both primary as well as secondary antibodies for my protein of interest , what else i need irrespective of RIPA buffer and protein G sluurry,
thanks
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