General Principles of Immunoprecipitation
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General Principles of Immunoprecipitation
This is the Bart's cookbook on immunoprecipitaiton. It gives some general principles on performing immunoprecipitation using different lysis buffers.(Sefton Lab, Salk Institute).
Content:
RIPA buffer
NP40 buffer
PBS
TN
Boiling in SDS
Antisera
Bugs
Secondary Antibodies
Cell Lysis
Pre-clearing
Tamara' s abbreviated protocol
Archived page
Last update 08-Feb-2006, Rating Poor of 7 votes.
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dear sir
I want to know the RIPA buffer for extract cell protein
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dear sir,
i want to prepare presentain about immunoprecipitation, can you please provide me with good details and information /enemenation
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i wanted to know the principle behind ouchterlony's double immunodiffusion method.
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respected sir,
i was working for an cancer i planned to work under immunoprecipitation follow to this i also do immuno histochemistry and western blotting as i am new to do this techniques can you please provide me the details and protocol about all these techniques with the materials.
thank you Rating: Very Good
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Dea sir,
I want to perform co immunopecipitation . can you please provide me with a good protocol and the details of the experiment?
Thanking you,
G.VIjay chaitanya Rating: n/a
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I am immunoprecipitating a phosphorylated enzyme, but in the negative control we get exactly the same strong band as in the sample. The negative control we use is where we do not add antibody to the beads, only the homogenate. Is this only due to non-specific binding or is it possible the homogenate precipitates and goes along with the beads all the time? Rating: n/a
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dear sir,
i am new to do immunoprecipitation, how can i do immunoprecipitation and then wetern blotting, i have both primary as well as secondary antibodies for my protein of interest , what else i need irrespective of RIPA buffer and protein G sluurry,
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