Technique / Proteomics and protein biochemistry / Immunoprecipitation

General Principles of Immunoprecipitation

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	By jack on 28-Jun-2007 dear sir I want to know the RIPA buffer for extract cell protein thanks Rating: n/a Reply

	By yasin daraweesh on 09-May-2007 dear sir, i want to prepare presentain about immunoprecipitation, can you please provide me with good details and information /enemenation thanks Rating: n/a Reply

	By Syeda Zainab Fatima on 04-Jan-2007 i wanted to know the principle behind ouchterlony's double immunodiffusion method. thanks Rating: n/a Reply

	By karthikeyan on 31-Oct-2006 respected sir, i was working for an cancer i planned to work under immunoprecipitation follow to this i also do immuno histochemistry and western blotting as i am new to do this techniques can you please provide me the details and protocol about all these techniques with the materials. thank you Rating: Very Good Reply

	By Vijay Chaitanya on 24-Jun-2006 Dea sir, I want to perform co immunopecipitation . can you please provide me with a good protocol and the details of the experiment? Thanking you, G.VIjay chaitanya Rating: n/a Reply

By J.Heerkens on 01-Mar-2006 I am immunoprecipitating a phosphorylated enzyme, but in the negative control we get exactly the same strong band as in the sample. The negative control we use is where we do not add antibody to the beads, only the homogenate. Is this only due to non-specific binding or is it possible the homogenate precipitates and goes along with the beads all the time? Rating: n/a Reply

	By pushpinder on 08-Feb-2006 dear sir, i am new to do immunoprecipitation, how can i do immunoprecipitation and then wetern blotting, i have both primary as well as secondary antibodies for my protein of interest , what else i need irrespective of RIPA buffer and protein G sluurry, thanks Rating: n/a Reply