Technique / Molecular Biology / miRNA microRNA
MicroRNA cloning protocol
MicroRNA cloning protocol
MicroRNA (miRNA) cloning protocol from Dr.Victor Ambros's lab at Dartmouth medical college.
Section I RNA ligation of 3` linker.
Section II. Gel purification of (RNA-to-3’ linker) ligation product.
Section III. RNA ligation of 5’ linker (optional, “Nelson” protocol).
Section IV. Reverse transcription
Section V. PCR amplification of cDNA
Section VI. Gel purification of cDNA
SectionVII. Cloning single-insert cDNAs.
Section VIII. Cloning Concatamerized cDNAs.
Section IX. Tailing of the ends of concatamers for T-vector ligation
Last update 12-Dec-2007, Rating of 1 votes.
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By karn pratap singh
on 12-Dec-2007
we have started the the same work so please give me protocol for successful research |
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