Technique / Pharmacology and Toxicology / Enzyme assays


CYTOCHROME P450 ASSAY



CYTOCHROME P450 ASSAY

Recombinant Enzyme/Microsome Protocol & References from Research diagnostics inc.

Last update 29-Jan-2008, Rating of 2 votes.

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By Baset on 25-Feb-2008
There are so many ways to measure CYP450 activity and that depends on the facilities you have such as HPLC, spectrophotometer .. etc. Methos of Omura and Sato can measure the overall CYP conc. but you can also measure individual isozymes by other utilizing specific substrate (e.g., p-nitro phenol for CYP 2C9).
Look in Methods in Enzymology Volume 272, Part B, 1996.
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By Pavan Kr. Singh on 29-Jan-2008
Sir, myself pavan kumar as research student and doing work on devlopmental stages of fish using molecular technique such as RAPD,but I wish used biochemical parameter as cytochromeP450. so please give me protocol for this.please help me.
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