Technique / Proteomics and protein biochemistry / protein gel staining and protein digestion for mass spec


What if silver stain does not work?



What if silver stain does not work?

Our PAGE gels Sometimes stain completely and you get
a "chrome-plated" surface with no trace of DNA bands. This happens with
different operators and everyone keeps his/her own stock solutions. The
AgNO3 we have is a little old, and so is the nonbinding silane, but
most of the time we get a good result.

The protocol we use is: ... ...

Last update 05-Jan-2004, Rating of 3 votes.

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By riyaz on 14-Jul-2007
I am also facing same prob , pls suggest me, how can i get good results in silver stain, which i am doing to detect DNA polymorphism (SSCP), i get very good PCR products , silver staining is creating all the possible problems
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By Andres on 12-Dec-2006
Hi we are working with ISSRs markers but our gels in agarose are fine, I mean the PCR products, but when we load the samples in denaturants gels for silver stain, some times we don't see the bands or in other ocassions we soa a smear on the bands. what is wrong in this case? thanks for your help
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By tes on 13-Oct-2006
I to am having the same problem, i have changed all solutions several times. I do not de gas or filter acrylamide, is it the reason for the faint bands. my PCR and electrophoresis is fine, since i can see very good fine bands, though faint
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By Aleya on 05-Jan-2004
We use silver staining to detect polymorphisms between our DNA samples, but recently the bands appear very faint although it is obvious that PCR is okay. How can we increase sensitivity so that the bands are more distinct and not appear as 'ghost' bands?
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