Overview of Antibody Production and tips

Overview of Antibody Production and tips by AnaSpec, Inc.

Content:

Introduction
Antigen Selection
Synthetic Peptides as Antigens
Selection of Animals
Sampling Serum
Complement Inactivation
Storage of Antibodies
References

Tips for Antibody Production



Protocol for ELISA Titration of Anti-Serum

Affinity Purification Protocol

Cell Lysate Preparation Protocol

SDS-Polyacrylamide Gel Electrophoresis

Western Blot Protocol
Troubleshooting for Western Blot
Antibody Labeling
Biotinylation of Purified Antibody Protocol
Labeling Antibodies with Horseradish Peroxidase (HRP) Protocol
Labeling Antibodies with Alkaline Phosphatase (AP) Protocol
Labeling Antibodies with Fluorochromes Protocol
References

Last update 15-Nov-2006, Rating of 1 votes.

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	By ali maleki on 15-Nov-2006 antibody purification & application Rating: Reply

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