Peptide antisera troubleshooting

A troubleshooting guide on antisera production using peptide antigen. (From Sigma-Genosys Canada).

Content:

My serum looks cloudy, is it okay?
I have received my sera and they are red in color. What is this and do I need to be concerned?
I am not seeing anything on my Western Blot. What do you recommend?
I am seeing a lot of bands (high background) on my western blot. What do you recommend?
My custom antiserum does not work in immunocytochemistry. What do you recommend?
I need to attach a peptide to a chromatography resin for affinity purification of antisera or proteins. What activated affinity resin should I use?
What should I do to terminate the project?

Last update 11-Jul-2007, Rating of 1 votes.

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	By ANIL KUMAR CHAVA on 11-Jul-2007 We have an issue with the antibody generated against custom synthesized peptide,its not recognising the right molecular size protein which supposed.We have taken all the precautions to avoid the clevage by the addition of Protease inhibitors.Any one has clue about this. Thanks Anil Rating: Reply

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