Technique / Histology / In situ hybridization
Probe synthesis for in situ hyb
Probe synthesis for in situ hyb
This is essentially according to the method recommended by Boehringer Mannheim. Cut 20 ug of plasmid DNA with appropriate enzyme in 100 ul. Pheno1, phenol/chloroform, chloroform and ethanol precipitate. Dissolve in 15 ul ddH20.(Hiroyuki Takeda lab, National institute of Genetics).
Last update 14-Apr-2005, Rating of 0 votes.
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