Prepare competent cells with RbCl2 method

PDF protocol from Marimoto lab at Northwestern University.

The procedure can be used to obtain competent cells with a transformation frequency of 10^6 - 10^7 colonies per microgram of DNA. Conveniently, these cells can be stored for months with relatively no loss in efficiency.

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Last update 23-Apr-2005, Rating n/a of 0 votes.