Technique / Molecular Biology / Bacterial plasmid bacterial phages vectors
Prepare competent cells with RbCl2 method
Prepare competent cells with RbCl2 method
PDF protocol from Marimoto lab at Northwestern University.
The procedure can be used to obtain competent cells with a transformation frequency of 10^6 - 10^7 colonies per microgram of DNA. Conveniently, these cells can be stored for months with relatively no loss in efficiency.
Last update 23-Apr-2005, Rating of 0 votes.
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