General guidance on tissue cell culture

I am working on corneal epithelial stem cells. The problem I am facing during culture is bacterial contamination due to this either growth become static or I have to through the culture. Basically I am doing primary culture for this frist I wash the sample with 1% betadine for 5 mins then antibiotic solution for 5 min and at last with 0.3% gatifloxacin. I am also using gentamicin and penicillin- streptomycin in the culture but still not able to eliminate bacterial contamination. Please help me regarding this.
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qhat is the principle behind using 5%co2 in tissue culture incubators
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I'm working on culturing mammalian cell esp in anti-TNF cell and anti-IFN cell.Now I want to ask about what is the optimized speed when we centrifuge to separate almostly all the cells?
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