Technique / Molecular Biology / RNA transcriptional and post-transcriptional regulation


DNA contamination in RNA sample



DNA contamination in RNA sample

I'm trying to look for a efficient method to remove the DNA contamination in my total RNA samples. I'm working with a Gram-positive bacteria E. hirae and I'm trying to use the RNA for quantitative RT-PCR.

I've used both the Gaunidium thiocyanate method and the Qiagen's RNeasy kit to extract my RNA. ... ...

Last update 29-Sep-2008, Rating of 1 votes.

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By Dulan on 29-Sep-2008
TRy to use Guiso thiocyanate with Sized fractionated silica. This will give you high quality RNA minus potential PCR inhibitors.. We have used it to isolate many viral RNAs and I am sure it will work with your Bacti RNA.

Refer the paper by Chungue et al on RNA isolation of dengue viruses (silica based method).

I may be able to send you a protocol which you could try out if necessary. This method is cost effective as well.
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