Gene construction advice needed

I'm looking for advice on a gene construction project. I'm trying to
assemble a 219 bp gene from oligonucleotides. I designed the oligos so
that each overlaps the next by ten complementary bases. The plan then is
to fill the gaps, then ligate. I can get perfect results with any two
adjacent oligos, but when ... ...

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Last update 26-Jun-2006, Rating n/a of 1 votes.