Telomere measurement by quantitative PCR

A free accessible original paper on telomere measurement by quantitative PCR.

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Articles by Cawthon, R. M.

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Polymorphism/mutation detection

DNA characterisation

Nucleic Acids Research, 2002, Vol. 30, No. 10

Richard M. Cawthon
Department of Human Genetics, University of Utah

It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging.

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