Expression microarray reproducibility is improved by optimising purification steps in RNA amplificat

A BMC Genomics open access paper on optimising purification steps in RNA amplification and labelling. (BMC Genomics)

Reference:

Expression microarrays have evolved into a powerful tool with great potential for clinical application and therefore reliability of data is essential. RNA amplification is used when the amount of starting material is scarce, as is frequently the case with clinical samples. Purification steps are critical in RNA amplification and labelling protocols, and there is a lack of sufficient data to validate and optimise the process.

Ali Naderi1 , Ahmed A Ahmed1 , Nuno L Barbosa-Morais1, 2 , Samuel Aparicio1 , James D Brenton1 and Carlos Caldas1
1Cancer Genomics Program, Department of Oncology, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 2XZ, United Kingdom
2Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, 1649-028 Lisbon, Portugal
BMC Genomics 2004, 5:9     doi:10.1186/1471-2164-5-9

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