Technique / Proteomics and protein biochemistry / protein gel staining and protein digestion for mass spec

Coomassie Blue: destaining


Coomassie Blue: destaining (archive)

I am using PhastGel BlueR coomassie blue dye from Pharmacia, I am concearned
with destaining the gel without losing the signal from the bands (I have
around 10ng protein per band)


I am currently destaining in 10% Acetic acid, this takes quite a long time
and leaves an uneven blue background. I was considering using methanol ... ...

Last update 07-Nov-2007, Rating Good of 1 votes.

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By Ayman on 07-Nov-2007
For 1 liter: 500mL methonal, 400mL water, and 100mL acetic acid.
Rating: Good

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