Technique / Proteomics and protein biochemistry / protein gel staining and protein digestion for mass spec
Coomassie Blue: destaining
Coomassie Blue: destaining
I am using PhastGel BlueR coomassie blue dye from Pharmacia, I am concearned
with destaining the gel without losing the signal from the bands (I have
around 10ng protein per band)
I am currently destaining in 10% Acetic acid, this takes quite a long time
and leaves an uneven blue background. I was considering using methanol ... ...
Last update 07-Nov-2007, Rating of 1 votes.
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