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Glycoproteins and IEF gels
Posted by: Halleys_Comet (IP Hidden, New member, 1)
Date: February 3, 2006 12:20PM
Hi everyone,
I have a large glycoprotein (runs at 140KDa on a reduced denatured gel) about have of its apparent molecular weight is sugars. I produce this protein in Chinese Hamster Ovary cells K1 which should add Sialic acids to the protein. Hence, making the pI of the protein slightly acidic probably 4-5. However, I have run the gel 3 different times with the same protein and got three different answers. The first time the protein was in 0.3M NaCl, 50mM Citric Acid pH5 and the pI came out between 4-5 great I thought, I will be able to repeat this easily for my thesis. However, the second time I had changed the elution buffer on the size exclusion column to 1M NaCl, 50mM Hepes pH6.5 and this time the pI came out around 6-7.5. So this put me in a quandry is it following the buffering capacity of the buffers or is it, as some people have reported, being made more alkaline due to an increase in salt. So I decided to dialyse my protein into a 10x diltued IEF loading buffer (pH10.3) and then run the sample, therefore the salt or the buffer can't affect the result - however now it runs with a pI of 7-8.5. I don't know if this is correct or not. All the theoretical pI databases give the protein sequence (without sugars) a pI of around 7.75ish Can anyone help, this one of my last experiments, I want to finish this thesis soon, Any help would be gratefully recieved, Thanks
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