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Real Time PCR Primer Design
Posted by: LucianaCSU (IP Hidden, New member, 1)
Date: April 2, 2007 04:22PM

I'm trying to find software (preferably something available online) to design real time PCR primers and I'm overwhelmed by all the different options. What do you recommend to use?
Thanks!

 

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Re: Real Time PCR Primer Design
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: April 21, 2007 10:58AM

I would recommend to use the primerexpress from ABI to design pcr primers for real-time pcr. You need to use it together with other softwares/web tools because it does'nt provide functions like recognize intron/exon junctions etc.

 

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