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Tranfection of Non adherent murine lymphocytescells
Posted by: Paul wafula (IP Hidden, New member, 2)
Date: July 18, 2005 07:20AM

Hello,
I have been attempting to transfect murine lymphocytes using viral particles expressing my gene of interest tagged to GFP. The lymphocytes are non adherent.
I have attempted transfecting using polybrene but no success. Anyone has an idea on how to deal with this problem??
Kind regards,
paul

 

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