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Strange DNA contamination
Posted by: ak19l (IP Hidden, New member, 1)
Date: February 25, 2008 04:12AM

Hi,

I have had a mysterious DNA contamination again and again after plasmid transformation to DH5alpha E.coli cells.

I have checked that the contamination isn't in ligation, transformation, DNA purification or restriction reagents, component cells, loading buffer or water. But from time to time strange ~2500 bp and ~4000 bp bands appear in gel electrophoresis after analytical RE digestion. The sizes are the same regardless of RE I use, brightness of the bands varies.

Do you have any ideas what causes this and how can I get rid of it?

Thanks!

 

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