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    <title>Biowww biotechniques forums</title>
    <link>http://www.biowww.net/forums/index.php</link>
    <description>Latest 20 threads of all forums</description>
    <copyright>Copyright(C) Biowww biotechniques forums</copyright>
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    <lastBuildDate>Thu, 17 May 2012 13:05:25 +0000</lastBuildDate>
    <ttl>60</ttl>
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      <title>Biowww biotechniques forums</title>
      <link>http://www.biowww.net/forums/</link>
    </image>
    <item>
      <title>Latest USMLE Books Step 1 on reasonably price</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1379</link>
      <description><![CDATA[USMLE Step 1 Books - Premium Package
Kaplan Step 1 Lecture Notes 2011
Kalpan Q Book Step 1 2011
Kaplan USMLE Medical Ethics 2nd edition 2010
Battle Price $ 90


To Order, Please visit usmleworldwide.com]]></description>
      <category>Basic laboratory techniques forum</category>
      <author>drjhonyabbie</author>
      <pubDate>Mon, 14 May 2012 10:04:28 +0000</pubDate>
    </item>
    <item>
      <title>All High yield on USMLE</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1378</link>
      <description><![CDATA[I have some really high yield usmle study materials that helped me do really good on my step 1 exam. If any1 is interested I will be happy to pass it to them. Please email me at 
Good Luck]]></description>
      <category>Biochemistry and Molecular biology</category>
      <author>rony_2012</author>
      <pubDate>Sun, 13 May 2012 10:35:10 +0000</pubDate>
    </item>
    <item>
      <title>microRNA in situ hybridization protocol:  zebrafish embryo &amp; adult</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1372</link>
      <description><![CDATA[Lagendijk AK, Moulton JD, Bakkers J.   doi:10.1242/bio.2012810]]></description>
      <category>siRNA and microRNA forum</category>
      <author>jonmoulton</author>
      <pubDate>Fri, 27 Apr 2012 15:35:10 +0000</pubDate>
    </item>
    <item>
      <title>problems with CHEF/PFGE</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1365</link>
      <description><![CDATA[My lab needs help with CHEF gels (separation of entire chromosomes). We are having constant problems with CHEF for the last two years. Before that, we never had problems for 6 years. We have Bio-RAD DRII system. The problem is that yeast chromosomes look]]></description>
      <category>Electrophoresis forum</category>
      <author>amalkova</author>
      <pubDate>Mon, 23 Apr 2012 03:10:54 +0000</pubDate>
    </item>
    <item>
      <title>Cs-glutamate</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1346</link>
      <description><![CDATA[I am trying to get my electrophysiology solutions ready for experiment. Most papers suggest CS-glutamate. I cannot find any info on this. Is there a protocol on how to make up this solution? Is it something that must be purchased? Any advice will be helpf]]></description>
      <category>Biophysics and electrophysiology forum</category>
      <author>zeedsy</author>
      <pubDate>Wed, 04 Apr 2012 18:46:08 +0000</pubDate>
    </item>
    <item>
      <title>Alkaline hydrolysis &amp; immunodetection</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1288</link>
      <description><![CDATA[protocol using for in situ staining involves 3M NaOH, which as I understand causes hydrolysis of proteins &amp; I suspect the reason have not been able to get immunocytochemistry or western blotting to work. Hoping that someone has recommendation on how t]]></description>
      <category>Proteomics and protein biochemistry forum</category>
      <author>Larispoli</author>
      <pubDate>Sat, 10 Mar 2012 04:15:24 +0000</pubDate>
    </item>
    <item>
      <title>Drying down a PCR mastermix</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1278</link>
      <description><![CDATA[Hello!

My work will focus on the pre-storage of a PCR mastermix on a microfluidic cartridge. I have no experience with drying or lyophilization and I didn't find any useful protocol for that.

Can anybody help me? How long can be the mastermix stored]]></description>
      <category>PCR and real-time PCR techniques forum</category>
      <author>StefanoR</author>
      <pubDate>Fri, 09 Mar 2012 09:39:22 +0000</pubDate>
    </item>
    <item>
      <title>hnRNP K is Required for Optic Axon Regeneration in Xenopus laevis</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1277</link>
      <description><![CDATA[Liu Y, Yu H, Deaton SK, Szaro BG.  . J Neurosci. 2012;32(10):3563-3574. doi:10.1523/JNEUROSCI.5197-11.2012]]></description>
      <category>RNA techniques forum</category>
      <author>jonmoulton</author>
      <pubDate>Thu, 08 Mar 2012 23:36:20 +0000</pubDate>
    </item>
    <item>
      <title>troublshoot</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1276</link>
      <description><![CDATA[Hi all, 
I have a Rt-PCR to detect the survivin gene . I tried many time the protocol indicated in such paper but it dosent work. pls I need help in troublshooting the smear background and primer dimer.
thanks]]></description>
      <category>PCR and real-time PCR techniques forum</category>
      <author>hussein</author>
      <pubDate>Thu, 08 Mar 2012 10:18:21 +0000</pubDate>
    </item>
    <item>
      <title>miR-221 Req'd for Endothelial Tip Cell Behaviors in Vascular Dev'ment</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1260</link>
      <description><![CDATA[Nicoli S, Knyphausen CP, Zhu LJ, Lakshmanan A, Lawson ND. miR-221 Is Required for Endothelial Tip Cell Behaviors during Vascular Development. Dev Cell. 2012 Feb 14;22(2):418-29.

]]></description>
      <category>siRNA and microRNA forum</category>
      <author>jonmoulton</author>
      <pubDate>Thu, 23 Feb 2012 19:02:05 +0000</pubDate>
    </item>
    <item>
      <title>Protein mass spectrometry service</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1259</link>
      <description><![CDATA[Hi All!

Services All -based analyses are performed using nano-flow high performance liquid chromatography (nHPLC) interfaced via electrospray ionization (ESI) to dual pressure linear ion trap mass spectrometers (LTQ Velos). All data analyses are conduc]]></description>
      <category>Mass spectrometry forum</category>
      <author>swensson</author>
      <pubDate>Thu, 23 Feb 2012 09:31:11 +0000</pubDate>
    </item>
    <item>
      <title>bwasw</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1258</link>
      <description><![CDATA[bwasw

Hello,

I'm new to bioinformatics, and need help with bwasw.

I'm trying to align my sample illumina bacterial genome to an already published reference bacterial genome. The aim is to find out what genes are present in my sample genome, but m]]></description>
      <category>Bioinformatics forum</category>
      <author>bgansw</author>
      <pubDate>Mon, 20 Feb 2012 05:38:02 +0000</pubDate>
    </item>
    <item>
      <title>Advanced Morpholino Oligomers: A Novel Approach to Antiviral Therapy</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1257</link>
      <description><![CDATA[Warren TK, Shurtleff AC, Bavari S.  Advanced Morpholino Oligomers: A Novel Approach to Antiviral Therapy.  Antiviral Res. 2012;]]></description>
      <category>RNA techniques forum</category>
      <author>jonmoulton</author>
      <pubDate>Wed, 15 Feb 2012 17:46:52 +0000</pubDate>
    </item>
    <item>
      <title>Spinal Muscular Atrophy &amp; oligo treatment</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1256</link>
      <description><![CDATA[Oligo treatment extends life from ~14 days to ~100 days in an SMN1 knockout mouse model of Spinal Muscular Atrophy.  Dr. Arthur Burghes interviewed by Dr. Kevin Flanigan from the Center for Gene Therapy at Nationwide Children's Hospital in Columbus, Ohio.]]></description>
      <category>Neuroscience and neurobiology forum</category>
      <author>jonmoulton</author>
      <pubDate>Tue, 14 Feb 2012 18:51:33 +0000</pubDate>
    </item>
    <item>
      <title>miRNA's translational inhibition by deadenylation-independent mechanisms</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1255</link>
      <description><![CDATA[Mishima Y, Fukao A, Kishimoto T, Sakamoto H, Fujiwara T, Inoue K.  Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1104-9. Epub 2012 Jan 9.]]></description>
      <category>siRNA and microRNA forum</category>
      <author>jonmoulton</author>
      <pubDate>Mon, 13 Feb 2012 21:01:46 +0000</pubDate>
    </item>
    <item>
      <title>Paranormal, telepathy, one million price from James Randi, telepathic Vinko</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1254</link>
      <description><![CDATA[James Randi is offering 1 000 000 US$ to any person that can give evidence to paranormal.
Things like telepathy , moving and bending objects using brain power.
Over 50% of people believe in paranormal , some people like Vinko Rajic can use telepathy
al]]></description>
      <category>Neuroscience and neurobiology forum</category>
      <author>neurostudxxx</author>
      <pubDate>Thu, 09 Feb 2012 20:49:11 +0000</pubDate>
    </item>
    <item>
      <title>Paranormal, telepathy, one million price from James Randi, telepathic Vinko</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1253</link>
      <description><![CDATA[James Randi is offering 1 000 000 US$ to any person that can give evidence to paranormal.
Things like telepathy , moving and bending objects using brain power.
Over 50% of people believe in paranormal , some people like Vinko Rajic can use telepathy
al]]></description>
      <category>Physiology</category>
      <author>neurostudxxx</author>
      <pubDate>Thu, 09 Feb 2012 20:13:47 +0000</pubDate>
    </item>
    <item>
      <title>Kaplan Books Step 2 at Cheap rates</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1252</link>
      <description><![CDATA[Kalpan Lecture Notes 2009 USMLE Step 2
Kalpan USMLE Step 2 CK Q Books 2009
First Aid USMLE Step 2 CK 2009
Q Bank USMLE Step 2 2011 (6 Volume)
Battle Price $ 135

To Order, Please visi]]></description>
      <category>Physiology</category>
      <author>dranthony29</author>
      <pubDate>Mon, 06 Feb 2012 07:36:49 +0000</pubDate>
    </item>
    <item>
      <title>Kaplan Books Step 2 at Very Low Prices</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1251</link>
      <description><![CDATA[USMLE Step 1 Books - Complete Package
Kaplan Step 1 Lecture Notes 2011
Kalpan Q Book Step 1 2011
Q Bank Step 1 2011 (6 Volume)
Kaplan USMLE Medical Ethics 2nd edition 2010
Goljan Rapid Review Lecture notes 2010 for Step 1
Battle Price $ 150

To Or]]></description>
      <category>Behavior science</category>
      <author>dranthony</author>
      <pubDate>Fri, 03 Feb 2012 07:36:28 +0000</pubDate>
    </item>
    <item>
      <title>RATT annotation tool</title>
      <link>http://www.biowww.net/forums/viewthread.php?tid=1250</link>
      <description><![CDATA[Hello, 

I'm new to bioinformatics and am trying to use the RATT annotation tool to transfer annotation from an already sequenced bacterial strain to another very closely related one.

RATT has run successfully, but I'm not sure how to interpret the r]]></description>
      <category>Bioinformatics forum</category>
      <author>bgansw</author>
      <pubDate>Fri, 03 Feb 2012 02:15:00 +0000</pubDate>
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