http://biowww.net/ Sun, 13 Sep 2009 05:57:48 +0100 FeedCreator 1.7.2 http://biowww.net/methods/detail-1.html Nucleic Acids Research.1992:20(17):4667 ... BrennerS http://biowww.net/methods/detail-2.html DNA was successfully isolated from numerous Journal of Clinical Microbiology.2004:42(9):4293 ... WickesB. L. http://biowww.net/methods/detail-3.html The new generic Total Nucleic Acid Isolation kit improved the detection limit of a cytomegalovirus (CMV) PCR from 400 to 200 copies/ml. Sensitivity and specificity in 30 CMV-positive and 100 negative samples were 100%. Analytical performance was excellent. T ... Mueller-LantzschNikolaus http://biowww.net/methods/detail-4.html Broad-range amplification of bacterial DNA from clinical specimens has proved useful for the diagnosis of various bacterial infections, especially during antimicrobial treatment of the patient. Optimal sample processing protocols for diagnostic broad-range b ... JalavaJari http://biowww.net/methods/detail-5.html Since current studies indicate the possible involvement of Clinical and Diagnostic Laboratory Immunology.2001:8(3):499 ... YamamotoYoshimasa http://biowww.net/methods/detail-6.html Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by m ... HyungN I http://biowww.net/methods/detail-7.html The lack of simple and efficient methods for extraction of DNA from Nocardia spp. has hampered molecular manipulation of the DNA for diagnostic purposes. In the present study, a method for the rapid extraction of undegraded genomic nocardial DNA was establis ... ZlotnikH http://biowww.net/methods/detail-8.html Nucleic Acids Research.1996:24(8):1587 ... PfenningerM http://biowww.net/methods/detail-9.html Nucleic Acids Research.1995:23(24):5087 ... PhillipsR A http://biowww.net/methods/detail-10.html Nucleic Acids Research.1994:22(24):5506 ... KaldenJ R http://biowww.net/methods/detail-11.html Nucleic Acids Research.1993:21(14):3328 ... SchubertI http://biowww.net/methods/detail-12.html Nucleic Acids Research.1993:21(21):4985 ... HydeJ http://biowww.net/methods/detail-13.html Nucleic Acids Research.1993:21(10):2529 ... DasR H http://biowww.net/methods/detail-14.html We describe a rapid and general method for isolating DNA-binding proteins in high yield from purified nuclei of animal cells. The method has been tested for the isolation of a series of different DNA-binding activities including those of transcription factor ... WellauerP K http://biowww.net/methods/detail-15.html Nucleic Acids Research.1992:20(15):4101 ... HoK K http://biowww.net/methods/detail-16.html Nucleic Acids Research.1992:20(21):5852 ... MurrayJ A http://biowww.net/methods/detail-17.html Nucleic Acids Research.1992:20(9):2381 ... JohnM E http://biowww.net/methods/detail-18.html Nucleic Acids Research.1991:19(8):1955 ... de MiccoP http://biowww.net/methods/detail-19.html Nucleic Acids Research.1991:19(23):6656 ... RothJ A http://biowww.net/methods/detail-20.html Nucleic Acids Research.1991:19(10):2792 ... ChowdhuryK http://biowww.net/methods/detail-21.html Current efforts to map the human genome are focused on individual chromosomes or smaller regions and frequently rely on the use of somatic cell hybrids. We report the application of the polymerase chain reaction to direct amplification of human DNA from hybr ... CaskeyC T http://biowww.net/methods/detail-22.html Current efforts to map the human genome are focused on individual chromosomes or smaller regions and frequently rely on the use of somatic cell hybrids. We report the application of the polymerase chain reaction to direct amplification of human DNA from hybr ... CaskeyC T http://biowww.net/methods/detail-23.html Nucleic Acids Research.1987:15(2):859 ... HildebrandC E http://biowww.net/methods/detail-24.html Nucleic Acids Research.1986:14(22):9220 ... BrickellP M http://biowww.net/methods/detail-25.html A method for rapid purification of plasmid DNA from lactic streptococci, utilizing microliter quantities of reagents, was developed by combination of a short lysozyme-mutanolysin cell wall digestion with a modification of the Escherichia coli plasmid isolati ... SandineW E http://biowww.net/methods/detail-26.html A method of isolating circular plasmid DNA from cleared lysates of E. coli is described. Purification is achieved by virtue of the rapid re-annealing kinetics or supercoiled DNA. After a brief denaturation step, double stranded plasmid DNA is separated from ... WilliamsJ G http://biowww.net/methods/detail-27.html A method of enriching for long double-stranded segments of eukaryotic DNA carrying particular genes is described. A purified RNA coded for by the gene is covalently attached to biotin via the protein, cytochrome c. This modified RNA is hybridized to total nu ... ManningJ http://biowww.net/methods/detail-28.html A procedure is reported for the isolation of cross-linked nucleosides from nitrous acid-treated calf thymus DNA. Cross-linked DNA was hydrolyzed enzymatically with deoxyribonuclease I and snake venom phosphodiesterase and fractionated on a DEAE-Sephadex colu ... ShapiroR http://biowww.net/methods/detail-29.html A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Cr ... StaffordD W http://biowww.net/methods/detail-30.html Proceedings of the National Academy of Sciences of the United States of America.1967:57(5):1514 ... VinogradJ http://biowww.net/methods/detail-32.html Nucleic Acids Research.1992:20(22):6115 ... GeigerH H http://biowww.net/methods/detail-33.html Nucleic Acids Research.1989:17(20):8393 ... TilzerL L http://biowww.net/methods/detail-34.html Nucleic Acids Research.1989:17(9):3604 ... PinckL http://biowww.net/methods/detail-35.html Nucleic Acids Research.1989:17(20):8390 ... EisenbergA http://biowww.net/methods/detail-36.html DNA purified from clinical cerebrospinal fluid and urine specimens by a silica-guanidiniumthiocyanate procedure frequently contained an inhibitor(s) of DNA-processing enzymes which may have been introduced by the purification procedure itself. Inhibition cou ... Wertheim-van DillenPauline http://biowww.net/methods/detail-37.html A novel subtraction hybridization procedure, incorporating a combination of four separation strategies, was developed to isolate unique DNA sequences from a strain of Rhizobium leguminosarum bv. trifolii. Sau3A-digested DNA from this strain, i.e., the probe ... CooperJ E http://biowww.net/methods/detail-38.html Nucleic Acids Research.1991:19(15):4293 ... BernsA http://biowww.net/methods/detail-39.html Nucleic Acids Research.1991:19(9):2507 ... HenleyD C http://biowww.net/methods/detail-40.html Nucleic Acids Research.1991:19(20):5792 ... MatsuuraS http://biowww.net/methods/detail-41.html Nucleic Acids Research.1990:18(21):6462 ... NathK http://biowww.net/methods/detail-42.html Nucleic Acids Research.1989:17(20):8390 ... EisenbergA http://biowww.net/methods/detail-43.html Nucleic Acids Research.1988:16(2):776 ... MagillJ M http://biowww.net/methods/detail-44.html A rapid and simple plasmid isolation procedure was developed for the epidemiological analysis of plasmid-mediated antimicrobial resistance. By this method, plasmid DNAs ranging in molecular weight between 2.0 and 122 X 10(6) could be detected. Various bacter ... NaganoY http://biowww.net/methods/detail-45.html Several plasmids, each containing a segment of DNA capable of conferring mitotic stability on the ars1 vector YRp7 replicating in yeast (Saccharomyces cerevisiae), have been isolated from a yeast genomic library by direct selection for stable plasmid mainten ... CarbonJ http://biowww.net/methods/detail-46.html Nucleic Acids Research.1989:17(13):5405 ... ClaverysJ P http://biowww.net/methods/detail-47.html Nucleic Acids Research.1988:16(21):10382 ... TamM F http://biowww.net/methods/detail-48.html Nucleic Acids Research.1987:15(22):9611 ... JeanpierreM http://biowww.net/methods/detail-49.html Nucleic Acids Research.1995:23(23):4930 ... SetoD http://biowww.net/methods/detail-50.html RNA-DNA chimeras, in which both DNA and RNA monomers are site-specifically substituted in the same strand, may be prepared only by chemical synthesis. Biochemical studies have revealed a number of surprising and subtle effects resulting from the insertion of ... RichA http://biowww.net/methods/detail-51.html A DNA isolation procedure was developed by using triple-helix formation and magnetic separation. In this procedure, target DNA is captured by a biotinylated oligonucleotide via intermolecular triplex formation, bound to streptavidin-coated magnetic beads, an ... CantorC R http://biowww.net/methods/detail-52.html Nucleic Acids Research.1991:19(19):5443 ... AdamA C http://biowww.net/methods/detail-53.html Nucleic Acids Research.1991:19(14):4003 ... MiuraK http://biowww.net/methods/detail-54.html Nucleic Acids Research.1988:16(21):10382 ... TamM F