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Diagnosis of mutations by the PCR double RFLP method (PCR-dRFLP).  full-text paper
Abstract: Nucleic Acids Research.1994:22(25):5773 ...

Partitioned pulsed-field gel electrophoresis-PCR (PPF-PCR): a new method for pulsed-field mapping fo  full-text paper
Abstract: Nucleic Acids Research.1994:22(9):1776 ...

PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction  full-text paper
Abstract: Nucleic Acids Research.1990:18(7):1920 ...

A simplified system for generating recombinant adenoviruses  full-text paper
Abstract: Duplex-PCR Gel electrophoresis for PCR products from Colony Duplex PCR with two primer sets AdFor/AdRev and KanaFor/KanaRev on AdEasy system generating two diagnostic fragments of 384 bp and 768 bp (see arrows) after homologous recombination.(N) negative contr ...

A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long g  full-text paper
Abstract: Nucleic Acids Research.2004:32(12):e98 ...

A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements  full-text paper
Abstract: We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed nucleotide elements (LINE). The PCR product, which represents ...

A simple two-step, ‘hit and fix’ method to generate subtle mutations in BACs using s  full-text paper
Abstract: The bacteriophage lambda recombination system has proven to be a valuable tool for engineering bacterial artificial chromosomes (BAC). Due to its high efficiency, subtle alterations in the BACs can be generated using oligonucleotides as targeting vectors. Sinc ...

DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis  full-text paper
Abstract: The availability of sequences of entire genomes has dramatically increased the number of protein targets, many of which will need to be overexpressed in cells other than the original source of DNA. Gene synthesis often provides a fast and economically efficien ...

RNA: a method to specifically inhibit PCR amplification of known members of a multigene family b  full-text paper
Abstract: The polymerase chain reaction (PCR) is a versatile method to amplify specific DNA with oligonucleotide primers. By designing degenerate PCR primers based on amino acid sequences that are highly conserved among all known gene family members, new members o ...

A simple and efficient method for PCR amplifiable DNA extraction from ancient bones  full-text paper
Abstract: DDBJ/EMBL/GenBank accession nos AF228540–AF228549Nucleic Acids Research.2000:28(12):e67 ...

CapSelect: a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-medi  full-text paper
Abstract: Here we present CapSelect as a novel experimental approach for the selective enrichment of full-length cDNAs in PCR-mediated analysis of mRNA sequences. The method combines the 5'-CAP-dependent addition of specifically three to four non-templated dCMP residues ...

New device and method for capture, reverse transcription and nested PCR in a single closed-tube.  full-text paper
Abstract: A device and improved method based on the use of a compartmentalized Eppendorf tube that allows capture, reverse transcription and nested-PCR in a single closed-tube has been developed and patented. The main advantages of the system are the high sensitivity ob ...

Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling  full-text paper
Abstract: The availability of comprehensive sets of genes has prompted the researchers to carry out systematic collection of gene expression data. RT–PCR has the highest specificity and sensitivity for transcript detection among all available methods. Low throug ...

Terminal transferase-dependent PCR: a versatile and sensitive method for in vivo footprinting and de  full-text paper
Abstract: We report here a new, sensitive and versatile genomic sequencing method, which can be used for in vivo footprinting and studies of DNA adducts. Starting with mammalian genomic DNA, single-stranded products are made by repeated primer extension; these products ...

Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.  full-text paper
Abstract: We present a rapid and efficient method for the detection of related transcripts with different expression levels. This approach combines the rapid amplification of cDNA ends (RACE) method with a cDNA subtractive technique. The strategy is based on successive ...

In vitro method for the generation of protein libraries using PCR amplification of a single DNA mole  full-text paper
Abstract: A novel in vitro method for the generation of a protein library has been developed using the polymerase chain reaction (PCR) amplification of a single DNA molecule followed by in vitro coupled transcription/translation. DNA template encoding green fluorescent ...

A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP  full-text paper
Abstract: Partial digestion of DNA fragments is a standard procedure for subcloning analysis and for generating restriction maps. We have developed a novel method to generate a partial digestion for any DNA fragment that can be amplified by PCR. The method involves the ...

Adaptor-tagged competitive PCR: a novel method for measuring relative gene expression.  full-text paper
Abstract: A simple and reliable PCR-based method to quantitate gene expression is described. Following the digestion of double-stranded cDNA by a restriction enzyme, an adaptor is ligated to a cDNA from a first RNA sample, and another adaptor to a second RNA sample. The ...

DOP-vector PCR: a method for rapid isolation and sequencing of insert termini from PAC clones.  full-text paper
Abstract: Nucleic Acids Research.1996:24(13):2614 ...

A rapid method for detecting specific amplified PCR fragments in microtiter plates.  full-text paper
Abstract: A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are ...

A simple and rapid electrophoresis method to detect sequence variation in PCR-amplified DNA fragment  full-text paper
Abstract: Nucleic Acids Research.1995:23(23):4928 ...

An improved PCR method for walking in uncloned genomic DNA.  full-text paper
Abstract: Nucleic Acids Research.1995:23(6):1087 ...

An improved ligase-free method for directional subcloning of PCR amplified DNA.  full-text paper
Abstract: Nucleic Acids Research.1995:23(6):1089 ...

A rapid and simple PCR-based method for isolation of cDNAs from differentially expressed genes.  full-text paper
Abstract: Recently two techniques have been reported which use arbitrarily primed RT-PCR amplification of cDNA fragments from subsets of mRNAs to detect cDNA fragments from differentially expressed mRNAs. Here we report a simple and rapid PCR-based protocol to both dete ...

Island rescue PCR: a rapid and efficient method for isolating transcribed sequences from yeast artif  full-text paper
Abstract: The identification of transcripts from large genomic regions cloned in yeast artificial chromosomes (YACs) or cosmids continues to be a critical and often rate-limiting step in positional cloning of human disease genes. We have developed a PCR-based method for ...

A simple method of preparing plant samples for PCR.  full-text paper
Abstract: Nucleic Acids Research.1993:21(17):4153 ...

An improved method of competitive PCR for quantitation of gene copy number.  full-text paper
Abstract: Nucleic Acids Research.1993:21(20):4848 ...

A rapid and versatile method to synthesize internal standards for competitive PCR.  full-text paper
Abstract: Nucleic Acids Research.1993:21(4):1047 ...

Overlapping PCR for Bidirectional PCR Amplification of Specific Alleles: A Rapid One-Tube Method for  full-text paper
Abstract: Rapid detection of single-base changes is fundamental to molecular medicine. PASA (Genome Research.1997:7(4):389 ...

An alternative, rapid method of plant DNA extraction for PCR analyses.  full-text paper
Abstract: Nucleic Acids Research.1992:20(17):4676 ...

A novel PCR method for amplifying exons (or genes) over intragenic (or intergenic) regions in the ge  full-text paper
Abstract: Nucleic Acids Research.1992:20(11):2903 ...

A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA.  full-text paper
Abstract: Nucleic Acids Research.1992:20(11):2900 ...

A reliable method for amplifying cDNA using the anchored-polymerase chain reaction (A-PCR).  full-text paper
Abstract: Nucleic Acids Research.1992:20(14):3793 ...

Improved method for direct PCR amplification from whole blood.  full-text paper
Abstract: Nucleic Acids Research.1992:20(24):6747 ...

Simplified method for confirmation of PCR products.  full-text paper
Abstract: Nucleic Acids Research.1991:19(16):4562 ...

A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.  full-text paper
Abstract: Nucleic Acids Research.1991:19(6):1349 ...

An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide.  full-text paper
Abstract: Nucleic Acids Research.1989:17(3):1266 ...

A rapid semi-automated microtiter plate method for analysis and sequencing by PCR from bacterial sto  full-text paper
Abstract: Nucleic Acids Research.1989:17(22):9498 ...

Post-PCR sterilization: a method to control carryover contamination for the polymerase chain reactio  full-text paper
Abstract: We describe a photochemical procedure for the sterilization of polynucleotides that are created by the Polymerase Chain Reaction (PCR). The procedure is based upon the blockage of Taq DNA polymerase when it encounters a photochemically modified base in a polyn ...


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