Methods / Real-time PCR applications
Dual-Probe Assay for Rapid Detection of Drug-Resistant
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Mutations in particular nucleotides of genes coding for drug targets or drug-converting enzymes lead to drug resistance in . For rapid detection of drug-resistant in clinical specimens, a simple and applicable method is needed. Eight TaqMan minor groove binder (MGB) probes, which discriminate one-base mismatches, were designed (dual-probe assay with four reaction tubes). The target of six MGB probes was the
Journal of Clinical Microbiology.2004:42(11):5277
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