Methods / General PCR applications
A rapid method for detecting specific amplified PCR fragments in microtiter plates.
<%urltitle%>
A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotides or oligonucleotides by using a positively charged DEAE cellulose matrix. Amplification products are visualized directly in the matrix using immunoenzymatic methods or streptavidin-conjugated enzymes. The detection process can be carried out within 2 h, allows the processing of large sample sizes and can potentially be automated.
Nucleic Acids Research.1996:24(16):3280
Last update 30-Nov-1999, Rating n/a of 0 votes.
Write your comment
Please Login or Register to Post
Diagnosis of mutations by the PCR double RFLP method (PCR-dRFLP).
Partitioned pulsed-field gel electrophoresis-PCR (PPF-PCR): a new method for pulsed-field mapping fo
PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction
A simplified system for generating recombinant adenoviruses
A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long g
A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements
A simple two-step, ‘hit and fix’ method to generate subtle mutations in BACs using s
DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis
RNA: a method to specifically inhibit PCR amplification of known members of a multigene family
b
A simple and efficient method for PCR amplifiable DNA extraction from ancient bones