Improved method for PCR-mediated site-directed mutagenesis.

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Nucleic Acids Research.1994:22(3):541

Last update 08-Feb-2007, Rating n/a of 1 votes.

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By hardeep on 08-Feb-2007
I want to join to sepratly amplified DNA fragments which has 45 base pair overlap, by PCR using overlap extension. but insted of getting the desired porduct of 3kb, I am getting lower molecular weight fragments, please help

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