Methods / DNA isolation and purification

A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers us


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Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape (Vitis spp.), apple (Malus spp.), pear (Pyrus spp.) and persimmon (Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis,Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8-2.0) routinely yielding 250-500 ng/microl (total 7.5-15 microg DNA from four to five tissue discs).

Nucleic Acids Research.1997:25(5):1085

Last update 30-Sep-2008, Rating n/a of 5 votes.

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By Radoslaw on 10-Nov-2006
Hello, i have problems witha herbal DNA isolation - green prepicitate. Standard method with CTAB. Please help me.

By Dr. R. B. Madariya on 18-Apr-2008
Problem in isolation of highly purified DNA from Jatropha curcas

By sunil on 27-Jul-2008
I want process of isolation of genomic dna from plant cell

By Deepika on 26-Feb-2008
Hello, I am having problem getting undegraded DNA using CTAB method from seabuckthorn. plz help me.

By suranjana on 30-Sep-2008
I have problems in doing PCR with my genomic DNA of Asparagus althouth a clear white precipitate is forming during isolation


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