Life science protocols and methods » PCR
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Competitive PCR (7)
Degenerate PCR (5)
GC-rich PCR (4)
in situ PCR (4)
Long PCR (7)
Methylation specific PCR (4)
Multiplex PCR (9)
Paraffin-Embedded Tissue PCR (7)
PCR primer design (23)
PCR purification (9)
RACE PCR (2)
Real-time PCR (39)
RT-PCR (9)
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| PCR carry-over contamination prevention PCR carry-over contamination prevention: use of dUTP instead of dTTP and treatment of PCR product with uracil-N-glycosylase (UNG) can prevent carry-over contaminations from previous pcr. |
| Hot start Hot start: one or more core component of PCR reaction are added at the final step when the optimal annealing temperature is reached.Vendors and products for hot-start PCR:AmpliWax PCR gems (Applied biosystems)PfuTurbo Hotstart (Stratagene)TaqStart Antibody (Clontech)AmpliTaq Gold (Applied biosystems)You can send let us know if your company has similar products. |
| DNA polymerase for PCR List of commercially available DNA polymerase:Taq (Thermus aquaticus)Pwo (Pyrococcus woesei)Pfu (Pyrococcus furiosus)rTth (Thermus thermophilus)Tfl (Thermas flavus)Tli/vent (Thermus litoris)Tma (Thermotoga maritima) |
