For quantifying mRNA, we use a competitive RT-PCR protocol with internal standard RNAs. These are added in a defined quantity to the RNA sample prior to the ...

Protocol for competitive RT-PCR protocol. From L 3226 ab of Dieter Kaufmann, Department of Human Genetics, University of Ulm, Germany. It describes procedure of ...

This protocol covers all stages in the competitive PCR and RT-PCR methods, from the design and construction of competitor molecules, and the competitive PCR ...

basis. Once the competitive internal standard has been constructed, no deviation is. required from the standard RT-PCR protocol in order to achieve accurate ...

Springer Protocols is the largest subscription-based electronic database of reproducible laboratory protocols in the Life and Biomedical Sciences.

Competitive PCR–ELISA combines competitive PCR with an ELISA to allow quantitative detection of PCR products. It is based on the inclusion of an internal ...

FIGURE 1 Construction of the YM standard for competitive PCR. The process is described in. detail in the text. ... tative PCR protocol, DNA from a normal ...