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CHO cell line culture

The Chinese Hamster Ovary (CHO) cell line is often used to express human recombinant glycoproteins. The CHO cell line can grow in both attached and suspension conditions. In suspension condition CHO cells grow in aggregates and thus make it easy for large-scale production of heterogenous recombinant proteins. <a href="http://www.atcc.org/common/catalog/numSearch/numResults.cfm?atccNum=CCL-61">CHO cell culture conditions from ATCC</a> <a href="http://stanxterm.aecom.yu.edu/wiki/index.php?page=Basic_CHO_cell_culture">Basic CHO cell culture</a>Improvement of CHO cell culture medium formulation: simultaneous substitution of glucose and glutamine. Altamirano C, Paredes C, Cairo JJ, Godia F. Biotechnol Prog. 2000 Jan-Feb;16(1):69-75.

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