Life science protocols and methods » Genetics » Ligase Chain Reaction
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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| Ligase chain reaction (LCR)--overview and applications. - http://www.ncbi.nlm.nih.gov/pubmed/8173509 Ligase chain reaction (LCR)--overview and applications. Wiedmann M, Wilson WJ, Czajka J, Luo J, Barany F, Batt CA. Department of Food Science, ... |
| Ligase chain reaction (LCR) definition - http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-L/ligase_chain_reaction.html Jul 26, 2004 ... LCR differs from PCR because it amplifies the probe molecule rather than producing amplicon through polymerization of nucleotides. ... |
| The ligase chain reaction in a PCR world. - http://genome.cshlp.org/content/1/1/5.full.pdf termed ligase chain reaction (LCR), to both amplify DNA and discriminate a single base mutation. (8-1 Although ... |
| Ligase chain reaction - Wikipedia, the free encyclopedia - http://en.wikipedia.org/wiki/Ligase_chain_reaction The Ligase chain reaction (LCR) is a method of DNA amplification. While the better-known PCR carries out the amplification by polymerizing ... |
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