Life science protocols and methods » Oligonucleotide » Oligonucleotides labeling
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| 3′-End Labeling of Oligonucleotide Protocol - http://www.springerprotocols.com/Full/doi/10.1385/0-89603-402-X:77?encCode=TkVHOjc3OlgtMjA0LTMwNjk4LTA= 3 Methods. 3.1 Oligonucleotide Labeling. This protocol allows the labeling of single-stranded oligonucleotides bearing a 3′-hydroxyl group. ... |
| Labelling oligonucleotides - http://www5.gelifesciences.com/aptrix/upp00919.nsf/content/B9F8085892682493C1256EB400417C99?OpenDocument&Path=Catalog&Hometitle=Catalog&entry=3&newrel&LinkParent=C1256FC4003AED40-B3A59FF465F49365C125701900490F61_RelatedLinksNew-AA6AFB057707B476C1257142007 Because of its ease of use and versatility there has been interest in the labelling of oligonucleotides. The following modified protocol has been used ... |
| Labeling of Synthetic Oligonucleotides Using the Klenow Fragment ... - http://cshprotocols.cshlp.org/cgi/content/full/2006/2/pdb.prot3577 Labeling of Synthetic Oligonucleotides Using the Klenow Fragment of E. coli DNA Polymerase I. Joseph Sambrook and David W. Russell. This protocol was ... |
| Oligonucleotide 3 -End Labeling With DIG-DdUTP, Biotin-DdUTP - http://protocols.biocompare.com/Protocols/ProtocolDetail/1161/Oligonucleotide-3'-End-Labeling-With-DIG-DdUTP,-Biotin-DdUTP,-Or-Fluorescein-DdUTP.html Details of this protocol, Oligonucleotide 3'-End Labeling With DIG-DdUTP, Biotin -DdUTP, Or Fluorescein-DdUTP, are located on a web site other than ... |
| A two-step general method for labeling of synthetic oligonucleotides - http://linkinghub.elsevier.com/retrieve/pii/S0968089606001234 A two-step general method for labeling of synthetic oligonucleotides is described. The protocol employs a cleavable universal linker, ... |
| Labeling Oligonucleotides and Nucleic Acids - http://www.ebiotrade.com/BUYF/productsf/Molecular%20Probes/sections/0802.htm Each Alexa Fluor Oligonucleotide Amine Labeling Kit provides the reagents required for three labelings of 100 µg each, a detailed protocol and ... |
| USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide - http://www.bio-medicine.org/biology-technology/-USB-OptiKinase-Eliminates-Base-Bias-in-Labeling-of-Oligonucleotide-5-Ends-1317-0/ The following protocol corresponds to use of OptiKinase for labeling of oligonucleotides by the forward reaction. The protocol is similar to that for T4 PNK ... |
| Nature Protocols: Spin labeling of oligonucleotides - http://www.natureprotocols.com/2007/05/24/spin_labeling_of_oligonucleoti.php Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances. In this protocol ... |